1. A tomato trichome stained with the specific fluorescent tonoplast marker MDY-64 and examined by confocal microscopy. (stills from movies) The upper panel shows a through-focus series at low magnification followed by a time lapse sequence at one plane of focus (speeded up 24x relative to real time). The lower panel is a time lapse sequence at higher magnification (same cell) to show that the channels seen in the preceding three movies are indeed delimited by tonoplast membrane (also 24x).

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