1. "Conventional" preparations for transmission electron microscopy often show a variety of artefactual distortions, though the general features are probably fairly true to life. This image comes from a superior mode of specimen preparation, namely high pressure freezing (which preserves the current state in milliseconds) followed by freeze-substitution. Note the smooth outline of the mitochondrial surface and the internal cristae. An arrow marks a connection between a crista and the inner envelope membrane. Mitochondrial ribosomes are clearly seen, and are smaller than their cytoplasmic counterparts. Eucalyptus sieberii root tip cell
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