Welcome to the GenAlEx 6.5 website!


GenAlEx: Genetic Analysis in Excel is a popular cross platform package for population genetic analysis that runs within Microsoft Excel. GenAlEx offers analysis of codominant, haploid and binary genetic loci and DNA sequences. Both frequency-based (F-statistics, heterozygosity, HWE, population assignment, relatedness) and distance-based (AMOVA, PCoA, Mantel tests, multivariate spatial autocorrelation) analyses are provided. In GenAlEx 6.5 we introduce exciting new features including calculation of new estimators of population structure: G’ST, G’’ST, Jost’s Dest, and F’ST via AMOVA, Shannon Information analysis, linkage disequilibrium analysis for biallelic data, and heterogeneity tests for spatial autocorrelation analysis. Direct data export is provided to more than 30 other software packages, and indirectly via common formats to many more packages. New teaching tutorials and extra step-by-step options are also included. The comprehensive independently written guide has been fully revised.

Go to Download to obtain GenAlEx 6.502 today!

GenAlEx 6.5 Overview

GenAlEx 6.5 (Peakall and Smouse 2006, 2012) offers a wide range of population genetic analysis options for the full spectrum of genetic markers within the Microsoft Excel environment on both PC and Macintosh computers. When combined with its user-friendly interface, rich graphical outputs for data exploration and publication, tools for data manipulation and export options to many other software packages, we believe that GenAlEx offers an ideal launching pad for population genetic analysis by students, teachers and researchers alike.

More About GenAlEx 6.5

Publications about GenAlEx

Peakall, R. and Smouse P.E. (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28, 2537-2539.

Freely available as an Open access article from here:


*Peakall, R. and Smouse P.E. (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6, 288-295.

*In Sep 2015, Google Scholar indicates the number of citations exceeds 7380 for GenAlEx 6 and more than 1200 for GenAlEx 6.5

Note that in 2013, GenAlEx 6.501 was released as a critical update for all GenAlEx users!  Please read the Release History and Read Me pdfs contained in the download pack for crucial information about the improvements and bug fixes.

GenAlEx 6.502 is now available!  This version offers new Shannon Diversity Analysis options as described in: Smouse, P.E., Whitehead, M.R., and Peakall, R. (2015) An informational diversity framework, illustrated with sexually deceptive orchids in early stages of speciation. Molecular Ecology Resources 15, 1375-1384 (DOI 10.111/1755-0998.12422)

Known issues for Apple users - Excel 2016 on Mac

Although early releases of Office 2016 are available for the Macintosh, current releases by Microsoft do not yet offer full Visual Basic for Applications (VBA) functionality, which is required to run GenAlEx within Excel.  Therefore, for the time being you will need to continue to run GenAlEx in Excel 2011.  Please note that this is a general issue for all VBA packages, and beyond our control.  We apologise for any inconvenience.  We will continue to monitor Microsoft releases and plan to provide a compatible version of GenAlEx as soon as possible.